This project aims to develop new particles and 3D structures with the ability to bind, like a natural antibody, to a biomolecule with high affinity and selectivity. These materials have high potential to overcome the drawbacks in biopurification and biosensors where natural molecules are used. The materials are extremely robust (plastic) thus in opposition to natural molecules no cold chain is needed, they can stand harsh conditions of pH, temperature ionic strength without losing affinity. In addition, they are cost effective since high affinity is obtained with very cheap polymers using a green technology. The project is leveraged on the previous background of the PI in the synthesis of molecularly imprinted polymers for API purification, which in combination with the members of the team from UCIBIO (expertise in proteomics) and the input of a company (CEV-Converd) to test the materials with real crude mixtures, the straight collaboration with Dr. Vasco Bonifácio (IST/ UL) organic chemist and Prof. Sergey Piletsky from the University of Leicester in a collaboration in computational simulation (SYBYL) will give a further step in the state of art by developing new cost-effective plastic antibodies for bio-recognition.